12/29/2023 0 Comments Macvector transloationIn elms, pathogenesis of the fungus Ophiostoma ulmi, the causative agent of Dutch elm disease (DED) is well understood as is the physiological response of the elm to infection. The techniques described in this patent application are applicable to the present invention. WO 90/07001 to Broglie et al describes DNA encoding chitinases for use in inhibiting fungi in detail. J., et al., Proc Natl Acad Sci USA 86:7895-7899 (1989)), conditions which could mimic cell death during an active infection. Finally, induction also results from wounding (Parsons, T. Ethylene, a gaseous plant hormone also produced during the course of a fungal infection, strongly induces chitinase activity (Boller, T., Oxford Surveys of Plant Molecular and Cell Biology 5:145-174 (1988) and Boller, T., et al., Planta 157:22-31 (1983)). This induction is also seen when plant tissue is treated with fungal cell wall material (Kurosaki, F., et al., Physiol Mol Plant Path 31:211-216 (1987) Roby, D., et al., Biochem Biophys Res Comm 143(3):885-892 (1987)). P., et al., Physiol Mol Plant Path 28:161-169 (1986) and Rasmussen, U., et al., Planta 187:328-334 (1992)) and mRNA encoding chitinase (Roby, D., et al., Plant Sci 52:175-185 (1987) Roby, D., et al., Physiological and Molecular Plant Pathology 33:409-417 (1988) and Meins, F., et al., Plant Cell 1:447-457 (1989)) are induced strongly during the course of a fungal infection. Accumulation of chitinase (Meins, F., Ahl, P., Plant Cell 61:155-161 (1989) Metraux, J. Several lines of evidence suggest strongly that chitinases are antifungal proteins. In particular the present invention relates to cDNA as set forth in SEQ ID NO:1.Ĭhitinases are pathogenesis response-related proteins found in a wide variety of plants. The present invention relates to a unique cDNA derived from a disease resistant hybrid Elm Tree which encodes a chitinase, a signal peptide and a sorting peptide. Sherald, et al., Canadian Journal of Forest Research, vol. Sticklen et al., Dutch Elm Disease Research pp. (1990) Methods for assessing the statistical significance of molecular sequence features by. Von Heijne, G., Sequence analysis in molecular biology: Treasure trove or trivial pursuit Academic Press, Inc. Biochem 133:17-21 (1983).Ĭhrispeels and Raikhel, The Plant Cell vol.
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